Fast Imaging Reveals Molecular Changes Involved in Vision

The structure of a crystallized protein changes as it reacts to light. The X-ray laser beam hits the crystal, diffracting photons, which allowed scientists to recreate a 3-D snapshot of the protein. Structural changes correspond to the protein's function (Source: SLAC)

The structure of a crystallized protein changes as it reacts to light. The X-ray laser beam hits the crystal, diffracting photons, which allowed scientists to recreate a 3-D snapshot of the protein. Structural changes correspond to the protein’s function (Source: SLAC)

Every process that sustains life is carried out by proteins, but under­standing how these complex molecules do their jobs depends on learning the arrange­ment of their atoms and how this structure changes as they react. No imaging method for observing molecular movement in such detail and speed had been available, until now.

A team of biochemists and physicists, led by the University of Wisconsin-Milwaukee and Imperial College London, have documented for the first time the funda­mental processes of a chemical reaction unfolding in real time. They captured images of a tiny crystalized protein as it reacted to light, 25 trillion per second. This allowed them build up a picture of what the protein was doing every few femtos­econds.

Previously, scientists had relied on X-ray crystallo­graphy, which could only take a static image of a protein. Now, they have been able to build up a series of crystallo­graphic snapshots into a molecular movie over extremely short timescales. Study co-author Jasper van Thor from Imperial’s Department of Life Sciences said: “Usually, we can only image the structure after the reaction, and infer what has happened. This is the first time we have been able to image crystal structures on timescales where the proteins are still undergoing the reaction. What happens during these timescales determines the outcome of the reaction, so knowing exactly what’s going on is vital. Previously our information and images of how the reactions work have been based on theory and spectros­copy. Now we can see it in reality.”

The team studied a yellow dye molecule at the centre of a light-sensitive protein that undergoes a shape shift as it interacts with a photon. The basic biological process they observed is similar to how the human eye’s retina responds to light. Using the Linac Coherent Light Source X-ray Free Electron Laser in California, the team fired intensely bright pulses at the protein, snapping images every few femto­seconds as the photon reaction progressed. Crucially, the result was enabled by careful tailoring of the visible light pulse, which is necessary with very bright and very brief pulses.

van Thor said: “We are working in interes­ting regimes that are new to crystallo­graphy, where the properties of the visible pulse matter most.” Next, the researchers will work on getting femto­second details in order to actively control the dynamics. This could ultimately allow scientists to intervene in the process of protein functions by using light. Now that the technique has been proven to work, the team hope it will start to be applied across molecular biology to unravel the mechanisms of all of proteins’ crucial reactions for life.

“This puts us dramatically closer to under­standing the chemistry necessary for all life,” said Marius Schmidt, physics professor at UW-Milwaukee and co-author of the paper. “Disco­vering the step-by-step process of how proteins function is necessary not only to inform treatment of disease, but also to shed light on the grand questions of biology.” Imperial’s van Thor is investi­gating the areas where this technology could potentially lead to new break­throughs, and is helping develop a case for a similar instrument facility to be built in the UK. (Source: ICL)

Reference: K. Pande et al.: Femtosecond structural dynamics drives the trans/cis isomerization in photoactive yellow protein, Science 352, 725, DOI: 10.1126/science.aad5081

Link: Centre for Free Electron Laser Science, Deutsches Elektronen Synchrotron, Hamburg, GermanyFaculty of Natural Sciences, Department of Life Sciences, Imperial College, London, UK

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